Gilbert, Sir John, eldest son of Sir Humphrey Gilbert, went with Raleigh to Guiana in 1595; showed gallantry at the battle of Cadiz in 1596 and was knighted by Essex; governor of the fort at Plymouth, 1597; member of the council for Virginia, 1607; was a brave officer; married a daughter of Sir Richard Molyneux, of Sefton, but died without issue, July 5, 1608, of small pox, and was buried at Marldon Church. His brother Raleigh Gilbert was his heir.