His will mentions:
-his wife Dame Margaret
-his sons Thomas, Francis, Robert, and Henry
-his daughters Dorothy and Martha
-his son-in-law Edmund Bedingforde Esquire and Anne his wife
-his servants Edward Giles, Bartholomew Pufany, Edmond Poole, Thomas Hollowye, Richard Giles, Besse Foolle, Robert Godden, William Roddenhurst, Humphrey Nevill, Nicholas Everard (and his wife Cecely),
-his wife's maids Dorothy Tuke(?), Susanne, Jane Stoneham,
-Lord and Lady Abergavenny
-his brothers Anthony Southwell, Sir Richard Southwell knight,
-relationship unspecified: John Hull; John Spowe(?) of Norwich;
Mr Anthony Weldon; Sir Henry Jernyngham, Knight; Sir Henry Bedingfelde, Knight; Sir Edmond Wyndham, Knight; William Aston; Thomas Swanne, gentleman, and William Swanne his son; William Smothinge; Mr George Dorrell of Calehill; Mr George Dorrell of Grayes Inn; Thomas Holte; Mr Richard Clerk; Henry Cuttlesynge(?); my lady Tyrrell wife of Nichas Cutler esquire;
-Geofferey Appriser(?), parson of Merewood
-John Thruston of Hoxon, esquire (one of his executors)
-his old friend Sir Nicholas Bacon, knight