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Matilda of Brandenburg (; b. ca. 1270 – d. bef. 1 June 1298), was a German princess member of House of Ascania and by marriage Duchess of Wrocław and High Duchess of Poland.
She was the second daughter of Otto V the Long, Margrave of Brandenburg-Salzwedel, by his wife Judith of Henneberg, daughter of Count Herman I, Count of Henneberg and heiress of Coburg and Schmalkalden.