Probably Jane died while her children were still growing up, date unknown, and Joseph married a second wife. This is learned from a surviving Bishop's Transcript of Layer marney of March 1639-March 1640. In this period, "Joseph Downing, an Infant, the sonne of Joseph Downing Clarke" was buried. Jane may have been too old to be the mother of this infant. On the other hand, this may have been her last child, after several others born in Layer Marney.