Members of the Walkers Creek Line trace their descent to John Walker of Wigton Scotland. They are included as part of Group 33 of the Walker YDNA project. (Some members of Group 33 trace their descent to Robert Walker of Letterkenny Ireland, a contemporary of John Walker I of Wigton, but whose relationship to John I is unknown.)
Group 8 of the Walker YDNA project also trace their descent to John Walker I of Wigton Scotland. These include persons traceing descent through Samuel Walker and Jane Patterson of Natural Bridge Virginia, who settled in the area by about 1750. White 1902 identifies Samuel as the grandson of John Walker I of Wigton Scotland.
At this time it is not clear whether Group 33 or Group 8 is in fact descended from John I. Additional research on this point is needed. Currently, the best case can be made for the Walkers Creek line being descended from John I of Wigton Scotland. Members of this group (specifically, John Walker III=Ann Houston), can be clearly documented to the Nottingham Lots in Old Chester, PA, living in an area now part of Cecil County MD. John Walker II=Katherine Rutherford, John III's parents per White 1902, are said to have lived in this same area. No members of Group 8 have been traced to the same area, so at this point it seems likely that John III is indeed a descendant of John I. Additional work, however, is needed. In particular, information on where Samuel Walker=Jane Patterson were living prior to their appearance in Natural Bridge, is needed.