Holybourne is a village in the East Hampshire district of Hampshire, England. It is 1.3 miles (2.2 km) northeast of the centre of Alton, is contiguous with it and shares the benefit of its A31 road bypass.
Alton railway station is 1.3 miles (2.2 km) southwest of the village.
The village has a population of around 1,500, and is also where Treloar College for physically handicapped secondary school pupils has one of its sites. Holybourne also has a pub - The White Hart - and a small store.
Holybourne is recorded in the Domesday Book of 1086 as Haliborne.
The name is thought to be derived from the Old English Haligburna which means sacred stream, referring to the small stream whose spring is near Holybourne Church whence it runs through the village.
English author Elizabeth Gaskell (1810–1865) bought a house in Holybourne in 1865. She died suddenly when visiting the house on 12 November 1865.
In the nineteenth century, a brewery was established in Holybourne by Walter Complin, who died in 1890. By the start of the 20th century, it was run by John Fowler Complin. The site is now occupied by a residential area called Complins.