Chastleton is a village and civil parish in the Cotswold Hills of Oxfordshire, England, about 4 miles (6.4 km) northeast of Stow-on-the-Wold. Chastleton is in the extreme northwest of Oxfordshire, on the boundaries with both Gloucestershire and Warwickshire. The 2011 UK census recorded the parish's population as 153.
Next to the parish church is Chastleton House, one of England's finest and most complete Jacobean houses. Completed in 1612, it has been occupied by members of the Jones family since 1602. It is now owned by The National Trust who opened the property to the public in 1997 after six years of conservation work. The house is full of objects accumulated by the family over the years: rare tapestries, portraits, furniture, as well as personal belongings, some just lying around, such as walking sticks and wellington boots. The gardens are typically Elizabethan and Jacobean, with a ring of topiary at their centre. The house is a Grade I listed building.