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Maria Manuela of Portugal (1527–1545; ) was a daughter of King John III of Portugal and his wife Catherine of Austria. She was Princess of Asturias as spouse of Philip, Prince of Asturias, and between 1527 and 1535 Princess of Portugal in her own right. Maria was born in Coimbra on 15 October 1527 and was one of the few children of John III to survive childhood. She married her double-cousin Prince Philip of Asturias (future Philip II of Spain and I of Portugal) in 1543 and was the mother of Prince Carlos, a deformed child who was heir of the Spanish crown. Maria died soon after she gave birth to Infante Carlos of Spain (1545–1568) on 12 August 1545 in Valladolid, Spain.
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